On January 28, 2021, Professor Yigong Shi's research team of Westlake University and Tsinghua University published a scientific research paper entitled "Structure of the activated human minor spliceosome" in Science journal, which is another major breakthrough in the study of spliceosome structure and mechanism.
In this paper, the team reported the high-resolution three-dimensional structure of the minor spliceosome for the first time, which is poorly understood so far, and showed a key conformation in the splicing reaction - activated minor spliceosome (defined as "minor Bact complex"), with an overall resolution up to 2.9Å.
This major breakthrough made Professor Yigong Shi's research team become the first team in the world to analyze the high-resolution three-dimensional structure of minor spliceosome, after first analyzing the world's first spliceosome structure in 2015 and the first human source spliceosome structure in 2017.
In this research work, GoodView™ from Beijing SBS Genetech Co Ltd was used in in vitro splicing assay and RT-PCR for the staining of nucleic acid. GoodView™ is a safer nucleic acid stain, an alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 268 nm and the other at 294 nm. In addition, it has one visible excitation at 491 nm. The Fluorescence emission of GoodView bound to DNA is centered at 530 nm. GoodView™ Nucleic Acid Stain is also included on New Products, Science Magazine, January 11, 2019.
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