On November 8, 2022, the research team led by Wang Mingwei from School of Basic Medicine, Fudan University, Peter K. Vogt from Scripps Research Institute, and Yang Dehua from Shanghai Institute of Materia Medica, Chinese Academy of Sciences, published a research paper entitled "Cryo-EM structures of cancer-specific helical and kinase domain mutations of PI3Kα" in the well-known journal PNAS (impact factor: 12.779).
This paper reports for the first time the freeze electron microscopic structure of three carcinogenic specific mutant (H1047R, E542K, and E545K) protein complexes of the human full-length phosphatidylinositol 3-kinase (PI3K) α subtype (p110α- p85α), clarifing the local and global conformational changes induced by mutants in PI3Kα production. This provides important information for in-depth understanding of the molecular mechanism of carcinogenesis of PI3Kα mutants and the creation of new anticancer drugs specifically targeting mutant PI3Kα.
In this research work, a site-directed mutagenesis kit from SBS Genetech was used. At SBS Genetech, we are at the forefront of offering tools for genetic engineering, from basic materials to complete solutions. SBS Genetech is also recognized as one of the key players in the global gene editing service market by third-party market researchers.
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